Effect of bee pollen extract on lead-induced toxicity in rat testis

Authors

  • Sofiane Bouazza Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria
  • Abbassia Demmouche Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria
  • Fawzia Toumi-Benali Laboratory of Spaces Eco-development, Djillali Liabes University, Sidi Bel Abbes 22000. Algeria.
  • Mouad Zouba Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria
  • Mohamed Rabie Bahri Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria
  • Nawel Agher Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria
  • Nacera Merakchi Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria
  • Mama El Ahmar Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

DOI:

https://doi.org/10.38150/sajeb.8(3).p91-102

Abstract

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;} @font-face {font-family:"Calibri Light"; panose-1:2 15 3 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 415 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1073786111 1 0 415 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:6.0pt; margin-left:0cm; text-align:justify; text-indent:21.25pt; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri Light"; mso-ascii-font-family:"Calibri Light"; mso-ascii-theme-font:major-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:"Calibri Light"; mso-hansi-theme-font:major-latin; mso-bidi-font-family:"Calibri Light"; mso-bidi-theme-font:major-latin; mso-ansi-language:EN-GB;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:FR;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:8.0pt; line-height:107%;} @page WordSection1 {size:612.0pt 792.0pt; margin:72.0pt 72.0pt 72.0pt 72.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} -->

Collected by honeybees, Bee pollen is a flower pollen gathered from selected flower species by special pollen traps, therapeutic qualities and reproductive performance of bee pollen are given from biologically active substances. Lead is a heavy metal, which induce reproductive intoxication, comes in the high levels from human activities.The aim of the present study was to evaluate the impact of bee pollen hydro-ethanolic extract on adult Wistar rat testis exposed to lead acetate sub-acute intoxication (15 days), highlighted using histological technics in situ. The biochemical and biometric measurement were conducted to verify any eventual toxicity of hydro-ethanolic extract in vivo. Animals were divided into four equal groups: first control group was treated orally with distillated water (1 ml) by gavage. The second group was administered 100 mg/kg/bw/day of a hydro-ethanolic extract of bee pollen, the third group was administered 30 mg/kg/bw/day lead acetate, the fourth group was given the both treatments in the same levels. The results showed that the relative organs weight indicates that only left kidney (p=0.001) and spleen (p=0.001) were attenuated without a considerable difference of the liver (p=0.07). the same was shown for relative weight of sexual organs. In addition, no significant difference, was marked, in serum biochemical parameters except for: Blood urea nitrogen, Alanine aminotransferase, Total bilirubin and Direct bilirubin. furthermore, ameliorative effect was shown after lead-induced alteration after histological stain of seminiferous tubules, indicating low lumen of seminiferous tubules with presence of spermatogonia and wide height of seminiferous epithelium. Biological activities of bee pollen extract, alleviate the lead induced toxicity on rat testis, marked by a complete spermatogenesis and prevention of Sertoli cells destruction.

Author Biographies

Sofiane Bouazza, Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria

Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria

Abbassia Demmouche, Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria

Laboratory of biotoxicology, Djillali Liabes University, Sidi Bel Abbes. 22000, Algeria

Fawzia Toumi-Benali, Laboratory of Spaces Eco-development, Djillali Liabes University, Sidi Bel Abbes 22000. Algeria.

Laboratory of Spaces Eco-development, Djillali Liabes University, Sidi Bel Abbes 22000, Algeria

Mouad Zouba, Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Mohamed Rabie Bahri, Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Nawel Agher, Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Nacera Merakchi, Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Mama El Ahmar, Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Department of Biology, Nature and life sciences faculty, Djillali Liabes University Sidi Bel Abbes. 22000, Algeria

Downloads

Published

2019-03-10

Issue

Section

Research Articles