Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

Authors

  • K. Zerhouni Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel AbbeÌ€s (Algeria)
  • B. Abbouni Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel AbbeÌ€s (Algeria)
  • K. Kanoun Faculty of Natural Science and Life, University of Djillali Liabes Sidi Bel AbbeÌ€s (Algeria)
  • K. Larbi Daouadji Laboratory of Applied Microbiology for industrial food in biomedical and the environment, Algeria
  • A. Tifrit Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel AbbeÌ€s (Algeria)
  • M. Benahmed Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)
  • T. M. Chaouche Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)

DOI:

https://doi.org/10.38150/sajeb.8(3).p76-82

Abstract

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

Author Biographies

K. Zerhouni, Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

B. Abbouni, Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

K. Kanoun, Faculty of Natural Science and Life, University of Djillali Liabes Sidi Bel Abbès (Algeria)

Faculty of Natural Science and Life, University of Djillali Liabes Sidi Bel Abbès (Algeria)

K. Larbi Daouadji, Laboratory of Applied Microbiology for industrial food in biomedical and the environment, Algeria

Laboratory of Applied Microbiology for industrial food in biomedical and the environment, Algeria

A. Tifrit, Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

Laboratory of Molecular Microbiology Proteomics and Health, Department of Biology, University of Djillali Liabes Sidi Bel Abbès (Algeria)

M. Benahmed, Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)

Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)

T. M. Chaouche, Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)

Department of Biology, Faculty of Natural Sciences and Life, University of Abou Bekr Belkaïd, Tlemcen (Algeria)

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Published

2019-03-10

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Section

Research Articles