MALDI-TOF analysis of seasonal dynamics of Lactic Acid Bacteria from Algerian Goat Milk suggests predominance of Enterococcus Genus

Authors

  • Nawal Benkrizi Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria
  • Abdelkader Homrani Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria
  • Ahmed Bekada Laboratory of Agronomy-Environment, Department of Biology, El-Wancharissi University Centre, Tissemsilt, Algeria
  • Naima Meghoufel Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria
  • Djilali Benbouziane Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis University, Mostaganem, Algeria
  • Fatima Zohra Adli Department of Biology, Abdelhamid Ibn Badis University, Mostaganem, Algeria

DOI:

https://doi.org/10.38150/sajeb.7(4).p173-180

Abstract

The goat milk is considered as nutraceutical due to its several beneficial com-pounds that increase its value. The purpose of this study was to investigate the variation of the goat milk’s lactic profile during winter and spring (2015). Several tests were realised. First, the isolation of the lactic acid bacteria con-tained in goat milk was realised by three specific media: the MRS, the M17, and the MSE. Second, a screening by the study of the proteolysis at two different concentration of casein (2%, 5%) and the study of aroma production were made to highlight performing isolates. These performing isolates were identified by using a precision spectrometry method which is called the Matrix Assisted Laser Desorption Ionisation Time-of-flight (MALDI-TOF). The identification with mass spectrometry «MALDI-TOF» technique revealed that those seasons had a more quantitative effect than a qualitative one. As a result, an average of 48% of lactic acid bacteria was identified for both sea-sons with a decrease of 6% observed in winter. In addition, it was revealed that Enterococcus was the most dominant genus in the Algerian goat milk. Therefore, lactic strains of this milk were proved to be technologically com-petent in terms of proteolysis and aroma production.

Author Biographies

Nawal Benkrizi, Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Abdelkader Homrani, Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Ahmed Bekada, Laboratory of Agronomy-Environment, Department of Biology, El-Wancharissi University Centre, Tissemsilt, Algeria

Laboratory of Agronomy-Environment, Department of Biology, El-Wancharissi University Centre, Tissemsilt, Algeria

Naima Meghoufel, Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Djilali Benbouziane, Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis University, Mostaganem, Algeria

Laboratory of Sciences and Animal Production Techniques, Department of Agronomy, Abdelhamid Ibn Badis Univer-sity, Mostaganem, Algeria

Fatima Zohra Adli, Department of Biology, Abdelhamid Ibn Badis University, Mostaganem, Algeria

Department of Biology, Abdelhamid Ibn Badis University, Mostaganem, Algeria

Downloads

Published

2018-05-17

Issue

Section

Research Articles