Butylated hydroxyanisole alleviates ferric nitrilotriacetate induced nephrotoxicity in rats by abrogation of oxidative stress

Abstract

In this study the effect of butylated hydroxyanisole (BHA), a phenolic antioxidant
used in food on Ferricâ€Nitrilotriacetate (Fe–NTA) induced nephrotoxicity is reported. Feâ€NTA (9 mg Fe/kg body weight, intraperitoneally) treatment enhanced the renal microsomal lipid peroxidation and hydrogen peroxide generation to ~2â€2.5 folds compared to salineâ€treated control and glutathione levels and the activities of antioxidant enzymes decreased to a range of 2–2.5 fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHA. Pretreatment with BHA prior to Fe†NTA treatment reduced microsomal lipid peroxidation and hydrogen peroxide generation to 1.3â€1.5 fold compared to control group and glutathione and the activities of antioxidant enzymes increased to a range of 1.5â€2 folds in kidney. Feâ€NTA administration enhanced value of blood urea nitrogen and creatinine to 3.7 and 2.5 fold respectively as compared to their corresponding control group. Administration of Feâ€NTA to rats receiving a pretreatment of BHA led to a significant diminution in both of these values. The results indicate that BHA is a potent chemopreventive agent and suppresses Feâ€NTA induced nephrotoxicity in rats.